澶у鍙互鎻愬墠 鍏嶈不(f猫i)闋�(y霉)绱勭煭淇℃彁閱�锛岀挵(hu谩n)鐞冪恫(w菐ng)鏍″皣鐐哄ぇ瀹剁櫦(f膩)甯�2021骞翠腑绱�(j铆)涓荤钘ュ斧鑰冭│鍏憡銆佽€冭│鍫�(b脿o)鑰冩浠剁瓑鏇村鍏�(n猫i)瀹�銆�

闂�(gu膩n)浜�2021骞翠腑绱�(j铆)涓荤钘ュ斧鑰冭│鍌欒€冭€冮粸(di菐n)“婊呰弻鍒跺姂鑸囩劇(w煤)鑿屽埗鍔�”鐨勫収(n猫i)瀹�锛岀挵(hu谩n)鐞冪恫(w菐ng)鏍″皬绶ㄧ偤澶у鏁寸悊濡備笅锛�

婊呰弻鍒跺姂鑸囩劇(w煤)鑿屽埗鍔�

涓€銆佹粎鑿岃垏鐒�(w煤)鑿屽埗鍔戝父鐢ㄧ殑鎶€琛�(sh霉)

(涓€)骞惧€�(g猫)鍩烘湰姒傚康锛�

1.婊呰弻锛氭婊呮垨闄ゅ幓娲荤殑寰敓鐗�(绻佹畺楂�+鑺借優)

2.娑堟瘨锛氭婊呮垨闄ゅ幓鐥呭師寰敓鐗�

3.闃茶厫(鎶戣弻)锛氭姂鍒跺井鐢熺墿鐨勭敓闀�(zh菐ng)鑸囩箒娈�

4.鐒�(w煤)鑿岋細鐗╅珨銆佷粙璩�(zh矛)銆佺挵(hu谩n)澧冧笉瀛樺湪浠讳綍娲荤殑寰敓鐗�

5.婊呰弻鍒跺姂锛氭婊呮垨闄ゅ幓鎵€鏈夋椿鐨勫井鐢熺墿绻佹畺楂斿拰鑺借優鐨勪竴椤炶棩鐗╁埗鍔�

6.鐒�(w煤)鑿屽埗鍔戯細閲囩敤鐒�(w煤)鑿屾妧琛�(sh霉)鍒跺倷鐨勪笉鍚换浣曟椿鐨勫井鐢熺墿绻佹畺楂斿拰鑺借優鐨勪竴椤炶棩鐗╁埗鍔�

(浜�)鐗╃悊婊呰弻鎶€琛�(sh霉)

1.骞茬啽婊呰弻娉�

鐩存帴鐏肩噿(閲戝爆銆佺幓鐠冨強鐡峰櫒-瀹夌摽180鈩� 1.5h)

骞茬啽绌烘埃婊呰弻娉�(娌规€ц粺鑶忓熀璩�(zh矛)銆佹敞灏勭敤娌�)

2.婵曠啽婊呰弻娉�——椋藉拰钂告苯/娌告按/娴侀€氳捀姹�

鍖呮嫭4椤烇細鐔卞銆佹祦閫氳捀姹�銆佺叜娌�銆佷綆婧枔姝�

褰遍熆鍥犵礌锛氬井鐢熺墿鐨勭ó椤炶垏鏁�(sh霉)閲�銆佽捀姹芥€ц唱(zh矛)銆佽棩鍝佹€ц唱(zh矛)銆佹粎鑿屾檪(sh铆)闁�銆佸叾浠�(浠嬭唱(zh矛)pH)

婵曠啽婊呰弻娉�

1)鐔卞婊呰弻娉�——鏈€鍙潬鐨勬粎鑿屾柟娉�

鐗归粸(di菐n)锛氶珮澹撻＝鍜屾按钂告苯鍔犵啽娈烘粎寰敓鐗�

閬╃敤锛氳€愰珮婧�銆佽€愰珮澹撹捀姹界殑钘ョ墿鍒跺姂銆佺幓鐠�/閲戝爆瀹瑰櫒銆佺摲鍣�銆佹鑶犲銆佹烤鑶滈亷(gu貌)婵惧櫒

鐔卞婊呰弻姊濅欢锛�

115鈩�×30min

121鈩�×20min

126鈩�×15min

F0鍊�(鐔卞婊呰弻鍙潬鎬у弮鏁�(sh霉)锛宮in)≥8…12

浣跨敤鐔卞婊呰弻鏌滄敞鎰忎簨闋�(xi脿ng)锛�

鈶犲繀闋堜娇鐢ㄩ＝鍜岃捀姹�;

鈶″繀闋堟帓鐩℃粎鑿屾煖鍏�(n猫i)绌烘埃;

鈶㈡粎鑿屾檪(sh铆)闁撲互鍏ㄩ儴钘ユ恫婧害閬�(d谩)鍒版墍瑕佹眰婧害鏅�(sh铆)闁嬪瑷�(j矛)鏅�(sh铆);

鈶ｆ粎鑿屽畬鐣㈠厛鍋滄鍔犵啽锛岄€愭几娓涘鑷冲鍔涜〃鎸囬嚌鐐�“0”鍚�锛屾斁鍑烘煖鍏�(n猫i)钂告苯锛屼娇鏌滃収(n猫i)澹撳姏鑸囧ぇ姘ｅ鐩哥瓑锛岀◢绋嶆墦闁嬫粎鑿屾煖锛�10锝�15min鍚庡叏閮ㄦ墦闁�銆備互鍏嶆煖鍏�(n猫i)澶栧鍔涘樊鍜屾韩搴﹀樊澶ぇ锛岄€犳垚琚粎鑿岀墿娌栧嚭鎴栫幓鐠冪摱鐐歌锛岀⒑淇濆畨鍏ㄧ敓鐢�(ch菐n)銆�

2)娴侀€氳捀姹芥粎鑿屾硶——100鈩冩祦閫氳捀姹斤紝30锝�60min

閬╃敤锛氭秷姣�銆佷笉鑰愰珮鐔卞埗鍔戞粎鑿�

3)鐓哺婊呰弻娉�——娌告按锛�30锝�60min

閬╃敤锛氭敞灏勫櫒銆佹敞灏勯嚌娑堟瘨

4)浣庢韩闁撴瓏婊呰弻娉�

灏囧緟婊呰弻鐗╃疆60锝�80鈩冪殑姘存垨娴侀€氳捀姹戒腑鍔犵啽60min娈烘粎绻佹畺楂�→鏀剧疆24h鑺借優鐧�(f膩)鑲叉垚绻佹畺楂�→鍐嶆鍔犵啽婊呰弻銆佹斁缃�→鍙嶅京(f霉)澶氭鐩磋嚦娈烘粎鎵€鏈夎娊鑳�

閬╃敤锛氫笉鑰愰珮婧�銆佺啽鏁忔劅鐗╂枡/鍒跺姂婊呰弻

3.閬�(gu貌)婵鹃櫎鑿屾硶——鐒�(w煤)鑿屾浠朵笅閫�(j矛n)琛屾搷浣�

閬╃敤锛氭埃楂�銆佺啽鏁忚棩娑�/鍘熸枡

甯哥敤婵惧櫒(瀛斿緫灏忎簬鑺借優楂旂⿳)

寰瓟婵捐啘婵惧櫒锛�0.22μm/0.3μm

鍨傜啍鐜荤拑婵惧櫒锛欸6

4.灏勭窔婊呰弻娉�

鈶犺蓟灏勬粎鑿屾硶锛�60Co銆�137Cs-γ灏勭窔

閬╃敤锛氱啽鏁�——缍敓绱犮€佹姉鐢熺礌銆佹縺绱�銆佺敓鐗╁埗鍝併€佷腑钘ユ潗鍜屼腑钘ュ埗鍔�銆侀啱(y墨)鐧傚櫒姊般€佽棩鐢ㄥ寘瑁濇潗鏂欏強钘ョ敤楂樺垎瀛愭潗鏂�

鈶″井娉㈡粎鑿屾硶——娑叉厠(t脿i)銆佸浐楂旂墿鏂�(鍏煎共鐕�)

鈶㈢传澶栫窔婊呰弻娉�——鏈€寮�(qi谩ng)254nm

閬╃敤锛氱墿楂旇〃闈�銆佺劇(w煤)鑿屽绌烘埃銆佽捀椁炬按

涓嶉仼浜庯細钘ユ恫銆佸浐楂旂墿鏂欐繁閮�

(涓�)鍖栧(xu茅)婊呰弻娉�

鈶犳埃楂旀粎鑿岋細姘ｆ厠(t脿i)娈鸿弻鍔�(鐠�(hu谩n)姘т箼鐑�銆佺敳閱�)

閬╃敤锛氱挵(hu谩n)澧冩秷姣�銆佷笉鑰愬姞鐔辩殑閱�(y墨)鐢ㄥ櫒鍏�/瑷�(sh猫)鍌�/瑷�(sh猫)鏂芥秷姣�銆佺矇鏈敞灏勫姂婊呰弻

鈶℃恫楂旀粎鑿屾硶

閬╃敤锛氱毊鑶�/鐒�(w煤)鑿屽櫒鍏�/瑷�(sh猫)鍌欐秷姣�

娈鸿弻鍔戯細75%涔欓唶銆�1%鑱氱董閰婧舵恫銆�0.1%-0.2%鑻墡婧撮姩(鏂版綌鐖炬粎)婧舵恫銆侀厷鎴栫叅閰氱殏婧舵恫

(鍥�)鐒�(w煤)鑿屾搷浣滄硶

1.鐗归粸(di菐n)锛氭暣鍊�(g猫)閬�(gu貌)绋嬫帶鍒跺湪鐒�(w煤)鑿屾浠朵笅閫�(j矛n)琛岋紝鐢�(ch菐n)鍝佷竴鑸笉鍐嶆粎鑿�锛岀壒娈婂彲鍐嶆粎鑿�(闈掗湁绱燝鑰愮啽)

2.閬╃敤锛氫笉鑰愮啽钘ョ墿鐨勬敞灏勫姂銆佺溂鐢ㄥ埗鍔�銆佺毊瑭︽恫銆佹捣缍垮姂鍜屽壍(chu脿ng)鍌峰埗鍔�

3.鍫�(ch菐ng)鎵€锛氱劇(w煤)鑿屾搷浣滃(鐢查啗婧舵恫鍔犵啽鐔忚捀娉�銆佺传澶栫窔婊呰弻銆佹恫楂旀粎鑿屾硶)銆佸堡娴佹綌鍑堝伐浣滆嚭(t谩i)銆佺劇(w煤)鑿屾搷浣滄煖

4.娉ㄦ剰锛氭墍鐢ㄤ竴鍒囩墿鍝併€佸櫒鍏峰強鐠�(hu谩n)澧冨潎闇€婊呰弻;鎿嶄綔浜哄摗鍏堟礂婢″苟鏇存彌婊呰弻琛ｉ瀷锛屼笉寰楀闇查牠鐧�(f膩)鍏�(n猫i)琛ｏ紝浠ュ厤姹℃煋銆�

(浜�)绌烘埃鍑堝寲鎶€琛�(sh霉)

1.绌烘埃瑾�(di脿o)绡€(ji茅)鎺柦——鍓�(chu脿ng)閫犳綌鍑堢┖姘�

宸ユキ(y猫)鍑堝寲(闄ゅ〉)銆佺敓鐗╁噲鍖�(闄ゅ〉銆侀櫎寰敓鐗�)

2.娼斿噲瀹ょ┖姘ｅ噲鍖栨(bi膩o)婧�(zh菙n)锛�2010鐗圙MPA銆丅銆丆銆丏绱�(j铆)

3.鐒�(w煤)鑿屾鏌ユ硶锛氱洿鎺ユ帴绋硶銆佽杽鑶滈亷(gu貌)婵炬硶(鍎�(y艒u)鍏�)

4.绌烘埃鍑堝寲鎶€琛�(sh霉)锛氱┖姘ｉ亷(gu貌)婵炬硶

浜�銆佹敞灏勫姂(灏忓閲忔敞灏勫姂)

(1)娉ㄥ皠鍔戠殑鍒嗛鍜岀郸钘ラ€斿緫

(2)娉ㄥ皠鍔戠殑鐗归粸(di菐n)鍜屼竴鑸唱(zh矛)閲忚姹�

(3)娉ㄥ皠鍔戠殑铏曟柟绲勫垎

(4)娉ㄥ皠鍔戠殑宸ヨ棟娴佺▼

(5)娉ㄥ皠鐢ㄦ按鐨勫埗鍌�

(6)鐔卞師

(7)娉ㄥ皠鍔戠殑鍒跺倷鑸囪唱(zh矛)閲忔鏌�

(8)鍏稿瀷娉ㄥ皠鍔戣檿鏂硅垏鍒跺倷宸ヨ棟鍒嗘瀽

(涓€)娉ㄥ皠鍔戠殑鍒嗛鍜岀郸钘ラ€斿緫

1.鍒嗛锛�

鈶犳憾娑插瀷锛氭按銆佹补

Vc銆佸畨涔冭繎銆佷簩宸颁笝閱�

鈶℃贩鎳稿瀷

閱嬮吀鍙殑鏉�銆侀瓪绮捐泲鐧借儼宄剁礌銆佸枩妯瑰牽

鈶钩鍔戝瀷

闈滆剤鐕�(y铆ng)椁�(y菐ng)鑴傝偑涔�

鈶ｆ敞灏勭敤鐒�(w煤)鑿岀矇鏈�(绮夐嚌)

闈掗湁绱犮€侀樋濂囬湁绱�銆佽泲鐧介叾椤�

2.娉ㄥ皠鍔戠殑绲﹁棩閫斿緫

鈶犵毊鍏�(n猫i)(琛ㄧ毊-鐪熺毊)锛�<0.2ml锛岀毊瑭�銆佺柧鐥呰ê鏂�

鈶＄毊涓�(鐪熺毊-鑲岃倝)锛�1锝�2ml锛屾按婧舵恫

鈶㈣倢鍏�(n猫i)锛�1锝�5ml;娌规憾娑�/娣锋嚫娑插欢鏁�銆佷钩鍔戝欢鏁�+闈跺悜

鈶ｉ潨鑴堬細闈滄帹銆侀潨婊�锛屽鐐烘按娑�锛屾补婧舵恫銆佹贩鎳告恫銆佷钩婵佹恫涓嶅疁闈滄敞浠ュ厤姣涚窗(x矛)琛€绠℃爴濉�

鈶よ剨妞庤厰锛�≤10ml锛岃垏鑴婃娑茬瓑婊�锛宲H5锝�8

鈶ュ嫊(d貌ng)鑴堬細瑷烘柗鐢ㄥ嫊(d貌ng)鑴堥€犲奖鍔�銆佽倽鍕�(d貌ng)鑴堟爴濉炲姂

鈶﹀叾浠栵細蹇冨収(n猫i)銆侀棞(gu膩n)绡€(ji茅)鍏�(n猫i)銆佹粦鑶滆厰鍏�(n猫i)銆佺┐浣�銆侀灅鍏�(n猫i)

(浜�)娉ㄥ皠鍔戠殑鐗归粸(di菐n)鍜屼竴鑸唱(zh矛)閲忚姹�

1.鐗归粸(di菐n)

鍎�(y艒u)榛�(di菐n)锛�

钘ユ晥杩呴€熴€佷綔鐢ㄥ彲闈�;鍙敤浜庝笉瀹滃彛鏈嶇郸钘ョ殑鎮ｈ€�;鍙敤浜庝笉瀹滃彛鏈嶇殑钘ョ墿;鐧�(f膩)鎻眬閮ㄥ畾浣嶄綔鐢�銆�

缂洪粸(di菐n)锛�

浣跨敤涓嶄究銆佹敞灏勭柤鐥�;瀹夊叏鎬т笉鍙婂彛鏈嶅埗鍔�;鍒堕€犻亷(gu貌)绋嬪京(f霉)闆滐紝鐢熺敘(ch菐n)鎴愭湰楂�(姘ｉ湩鍔�)锛屽児(ji脿)鏍艰純楂樸€�

2.娉ㄥ皠鍔戠殑涓€鑸唱(zh矛)閲忚姹�

鈶犵劇(w煤)鑿�

鈶＄劇(w煤)鐔卞師

鈶㈡緞鏄庡害(鍙(ji脿n)鐣扮墿)锛氫笉寰楁湁鑲夌溂鍙(ji脿n)鐨勬妇婵佹垨鐣扮墿

鈶ｅ畨鍏ㄦ€э細涓嶈兘寮曡捣灏�(du矛)绲勭箶鐨勫埡婵€鎬ф垨鐧�(f膩)鐢熸瘨鎬у弽鎳�(y墨ng)锛岀壒鍒ユ槸涓€浜涢潪姘存憾鍔戝強涓€浜涢檮鍔犲姂

鈶ゆ徊閫忓锛氳婕跨瓑婊叉垨绋嶉珮锛岄潨婊寸瓑寮�

鈶H锛�4锝�9(琛€娑�7.4)

鈶︾┅(w臎n)瀹氭€�

鈶ч檷澹撶墿璩�(zh矛)锛氬京(f霉)鏂规皑鍩洪吀娉ㄥ皠娑�

(涓�)娉ㄥ皠鍔戣檿鏂圭祫鎴�

娉ㄥ皠鐢ㄥ師鏂�

娉ㄥ皠鐢ㄦ憾鍔�(姘�銆佹补銆侀潪姘�)

娉ㄥ皠鐢ㄩ檮鍔犲姂

1.娉ㄥ皠鐢ㄦ按

璩�(zh矛)閲忚姹傦細鐒�(w煤)鑹�銆佺劇(w煤)鍛炽€佺劇(w煤)鑷�銆佹緞鏄�

pH5-7.绱�(x矛)鑿屽収(n猫i)姣掔礌<0.25EU/ml

2.娉ㄥ皠鐢ㄦ补(2015鐗堣棩鍏�)

妞嶇墿娌�(澶ц眴娌广€佽姖楹绘补銆佽尪娌�)銆佽嫰鐢查吀鑺愰叝銆佹补閰镐箼閰�

3.鍏朵粬娉ㄥ皠鐢ㄩ潪姘存憾鍔�

绗﹀悎娉ㄥ皠鐢ㄨ(gu墨)鏍硷紝涓嶈兘鐢ㄥ寲瀛�(xu茅)瑭﹀姂浠ｆ浛!

4.娉ㄥ皠鍔戠殑闄勫姞鍔�(浣滅敤銆佺ó椤�銆佸父鐢ㄥ搧绋�)

澧炲姞涓昏棩婧惰В搴﹀婧跺姂銆佹饯(r霉n)婵曞姂銆佷钩鍖栧姂鑱氬北姊ㄩ叝銆佽仛缍叜銆佽仛涔欎簩閱�-40銆佽摉楹绘补銆佸嵉纾疯剛銆� Pluronic F-68

闃叉涓昏棩姘у寲鎶楁哀鍔戜簽纭吀閳�銆佺～浠ｇ～閰搁垑

浜炵～閰告矮閳�銆佺劍浜炵～閰搁垑

鎯版€ф埃楂擭2銆丆O2

铻悎鍔戜箼浜岃兒鍥涗箼閰�(EDTA)銆丒DTA·2Na

鎶戝埗寰敓鐗╃敓闀�(zh菐ng)鎶戣弻鍔戠敳閰�銆佽嫰閰�銆佷笁姘彅涓侀唶銆佺～鏌虫睘

瑾�(di脿o)绡€(ji茅)pH(4-9)绶╂矕鍔戦唻閰�(閳�)銆佹灨娅為吀(閳�)銆侀厭鐭抽吀(閳�)銆佷钩閰搞€佺７閰告矮浜岄垑銆佺７閰镐簩姘垑銆佺⒊閰告矮閳�銆佺⒊閰搁垑

瑾�(di脿o)绡€(ji茅)婊查€忓婊查€忓瑾�(di脿o)绡€(ji茅)鍔慛aCl銆佽憽钀勭硸銆佺敇娌�

娓涜紩鐤肩棝灞€楹诲姂鍒╁鍗″洜銆侀菇閰告櫘榄崱鍥�銆佽嫰鐢查唶銆佷笁姘彅涓侀唶

5.娉ㄥ皠鍔戠殑绛夋徊鑸囩瓑寮佃(di脿o)绡€(ji茅)

绛夋徊婧舵恫锛氳垏琛€婕挎徊閫忓鐩哥瓑鐨勬憾娑�

绛夊嫉婧舵恫锛氭徊閫忓鑸囩磪绱�(x矛)鑳炶啘寮靛姏鐩哥瓑鐨勬憾娑�

娉ㄥ叆姗�(j墨)楂斿収(n猫i)鐨勬恫楂斾竴鑸姹傜瓑婊�锛屽惁鍓囨槗鐢�(ch菐n)鐢熷埡婵€鎬ф垨婧惰绛�銆�

0.9%姘寲閳夋憾娑诧細绛夋徊婧舵恫+绛夊嫉婧舵恫

婊查€忓鐨勮(di脿o)绡€(ji茅)锛氬啺榛�(di菐n)闄嶄綆鏁�(sh霉)鎿�(j霉)娉�銆佹隘鍖栭垑绛夋徊鐣�(d膩ng)閲忔硶

婊查€忓鐨勮(di脿o)绡€(ji茅)

鈶犲啺榛�(di菐n)闄嶄綆鏁�(sh霉)鎿�(j霉)娉�

W锛氶厤鎴�100ml绛夋徊婧舵恫鎵€闇€鍔犲叆绛夋徊瑾�(di脿o)绡€(ji茅)鍔戠殑閲�锛実/ml;

a锛氭湭缍�(j墨ng)瑾�(di脿o)鏁寸殑钘ョ墿婧舵恫寮曡捣鐨勫啺榛�(di菐n)涓嬮檷搴�

b锛�1%(g/ml)绛夋徊瑾�(di脿o)绡€(ji茅)鍔戞憾娑叉墍寮曡捣鐨勫啺榛�(di菐n)涓嬮檷搴�

1%姘寲閳夛細鍐伴粸(di菐n)闄嶄綆鐐�0.58鈩�锛屽繀闋堢墷瑷�!

(鍥�)娉ㄥ皠鍔戠殑宸ヨ棟娴佺▼

绺芥祦绋嬶細鍒舵按銆佸畨鐡垮墠铏曠悊銆侀厤鏂�銆佹垚鍝�

(浜�)娉ㄥ皠鐢ㄦ按鐨勫埗鍌�

1.鍘熸按铏曠悊

闆㈠瓙浜ゆ彌娉曘€侀浕婊叉瀽娉�銆佸弽婊查€忔硶

2.钂搁ぞ娉�——钘ュ吀娉�

3.鏀堕泦锛氱劇(w煤)鑿屾烤閬�(gu貌)瑁濈疆瀵嗛枆绯荤当(t菕ng)

4.璨瓨锛�80鈩冧互涓婁繚婧�

70鈩冧互涓婁繚婧惊鐠�(hu谩n)

4鈩冧互涓嬬劇(w煤)鑿岀媭鎱�(t脿i)瀛樻斁

瑕�(gu墨)瀹氭檪(sh铆)闁撳収(n猫i)浣跨敤锛�12h

(鍏�)鐔卞師

寰敓鐗╃敘(ch菐n)鐢熺殑鍏�(n猫i)姣掔礌

瀛樺湪浜庣窗(x矛)鑿岀殑绱�(x矛)鑳炶啘鍜屽浐楂旇啘涔嬮枔

鍏�(n猫i)姣掔礌=鐔卞師=鑴傚绯�

1.鐔卞師鐨勬€ц唱(zh矛)

鈶犺€愮啽鎬э細180鈩� 3锝�4h锛�200鈩� 60min锛�250鈩� 30锝�45min

鈶￠亷(gu貌)婵炬€э細1锝�5nm

鈶㈡按婧舵€�

鈶ｈ鍚搁檮鎬�

鈶や笉鎻櫦(f膩)鎬э細钂搁ぞ姘村櫒涓婇檮鏈夐殧娌缃�

鈶ュ叾浠栵細涓嶈€愬挤(qi谩ng)閰搞€佸挤(qi谩ng)鍫�銆佸挤(qi谩ng)姘у寲鍔戙€佽秴鑱叉尝銆佹煇浜涜〃闈㈡椿鎬у姂(濡傚幓姘ц喗閰搁垑)浣夸箣澶辨椿

2.鐔卞師鐨勬薄鏌撻€斿緫

鈶�?g貌u)娣栤懜婧嗙洘顔顬�?mdash;—涓昏渚�(l谩i)婧�

鈶″緸鍘熻紨鏂欎腑甯跺叆锛氱敓鐗╁埗钘�锛屽鍙虫棆绯栭厫銆佹姉鐢熺礌

鈶㈠緸瀹瑰櫒銆佺敤鍏�銆佺閬撳拰瑷�(sh猫)鍌欏付鍏�

鈶ｅ緸鍒跺倷閬�(gu貌)绋嬭垏鐢熺敘(ch菐n)鐠�(hu谩n)澧冧腑甯跺叆

鈶ゅ緸杓告恫鍣ㄥ叿甯跺叆

3.鐔卞師鐨勫幓闄ゆ柟娉�

鈶犲鍣ㄦ垨鐢ㄥ叿

鈼嗛珮婧硶

閲濋牠銆侀嚌绛�銆佺幓鐠�

250鈩�锛�30min

閰稿牽娉�

閲嶉壔閰搁墍纭吀娓呮礂娑�

绋€NaOH

鈶¤棩娑叉垨婧跺姂

鍚搁檮娉曪細娲绘€х偔

闆㈠瓙浜ゆ彌娉�

鍑濊啝閬�(gu貌)婵炬硶

鍙嶆徊閫忔硶锛氫笁閱嬮吀绾栫董鑶�

瓒呮烤娉曪細3-15nm瓒呮烤鑶�

鍏朵粬锛氬娆℃繒鐔辨粎鑿屻€佸井娉�

QIAN 鐔卞師鎬ц唱(zh矛)鍙婂幓闄ゆ柟娉�

鐔卞師鐨勫熀鏈€ц唱(zh矛) 鍘婚櫎鐔卞師鐨勬柟娉�

鑰愮啽鎬� 楂樻韩娉� 18034 2001 25030

姘存憾鎬�銆佹烤閬�(gu貌)鎬� 鍑濊啝婵鹃亷(gu貌)娉�銆佽秴婵炬硶銆佸弽婊查€忔硶

琚惛闄勬€� 鍚搁檮娉曘€侀洟瀛愪氦鎻涙硶

涓嶆彯鐧�(f膩)鎬� 钂搁ぞ姘村櫒涓婇檮鏈夐殧娌缃�

閰稿牽姘у寲鐮村 閰稿牽娉曪細閲嶉壔閰搁墍纭吀娓呮礂娑�銆佺█NaOH

(涓�)娉ㄥ皠鍔戠殑鍒跺倷鑸囪唱(zh矛)閲忔鏌�

绺芥祦绋嬶細鍒舵按銆佸畨鐡垮墠铏曠悊銆侀厤鏂�銆佹垚鍝�

鏂扮増GMP锛欰銆丅銆丆銆丏

1.娉ㄥ皠瀹瑰櫒鐨勮檿鐞�

瀹夌摽

涓€х幓鐠冿細涓€ц棩娑�銆佸急閰告€ц棩娑�-glc

鍚媷鐜荤拑锛氳€愬牽-纾鸿兒鍢у暥閳夋敞灏勬恫(pH10锝�10.5)

鍚嫰鐜荤拑锛氶吀鍫挎€у挤(qi谩ng)銆侀垑楣�

寮忔ǎ锛氭湁闋稿畨鐡�銆佺矇鏈畨鐡�銆佹洸闋告槗鎶樺畨鐡�

瑕�(gu墨)鏍硷細1.2.5.10銆�20ml

2.閰嶅埗鑸囬亷(gu貌)婵�

閰嶅埗锛氱█閰嶆硶(鍘熸枡璩�(zh矛)閲忓ソ涓€娆￠厤鎴�)銆佹績閰嶆硶

閬�(gu貌)婵撅細鍨傜啍鐜荤拑婵惧櫒:G2銆�3甯稿锛孏3銆�4娓涘/鍔犲锛孏5銆丟6銆�6鐒�(w煤)鑿�;寰瓟婵捐啘婵惧櫒

3.婊呰弻鑸囨婕�

娴侀€氳捀姹芥粎鑿岋細1锝�5ml瀹夌摽100鈩�銆�30min

10锝�20ml瀹夌摽100鈩冦€�45min

瑕佹眰鎸夋粎鑿屾晥鏋淔0澶т簬8閫�(j矛n)琛岄(y脿n)璀�銆�

4.娉ㄥ皠鍔戠殑璩�(zh矛)閲忔鏌�

绱�(x矛)鑿屽収(n猫i)姣掔礌鎴栫啽鍘熸鏌�

瀹跺厰娉曪細钘ュ吀娉�

楸熻│椹�(y脿n)娉�

(鍏�)鍏稿瀷娉ㄥ皠鍔戣檿鏂硅垏鍒跺倷宸ヨ棟鍒嗘瀽

1.缍敓绱燙娉ㄥ皠娑�(鎶楀琛€閰�)

銆愯檿鏂广€�

缍敓绱燙銆€銆€銆€(涓昏棩)銆€銆€銆€銆€ 104g

渚濆湴閰镐簩閳夈€€銆€(绲�(lu貌)鍚堝姂)銆€銆€銆€0.05g

纰抽吀姘垑銆€銆€銆€(pH瑾�(di脿o)绡€(ji茅)鍔�)銆€銆€49.0g

浜炵～閰告矮閳夈€€銆€(鎶楁哀鍔�)銆€銆€銆€2.0g

娉ㄥ皠鐢ㄦ按銆€銆€銆€ 鍔犺嚦銆€銆€銆€銆€銆€1000ml

缍敓绱燙娉ㄥ皠娑�(鎶楀琛€閰�)鍒跺倷宸ヨ棟锛�

鍦ㄩ厤鍒跺鍣ㄤ腑锛屽姞铏曟柟閲�80%鐨勬敞灏勭敤姘�锛岄€欳O2鑷抽＝鍜�锛屽姞VC婧惰В鍚�锛屽垎娆＄珐绶╁姞鍏ョ⒊閰告矮閳�锛屾敧鎷屼娇瀹屽叏婧惰В锛屽姞鍏ラ爯(y霉)鍏堥厤鍒跺ソ鐨勪緷鍦伴吀浜岄垑鍜屼簽纭吀姘垑婧舵恫锛屾敧鎷屽潎鍕�锛岃(di脿o)绡€(ji茅)钘ユ恫pH6.0锝�6.2锛屾坊鍔犱簩姘у寲纰抽＝鍜岀殑娉ㄥ皠鐢ㄦ按鑷宠冻閲�锛岀敤鍨傜啍鐜荤拑婕忔枟鑸囪啘婵惧櫒閬�(gu貌)婵撅紝婧舵恫涓€氫簩姘у寲纰�锛屽苟鍦ㄤ簩姘у寲纰�?x矛)楗（楣垮柧?锛屾渶鍚�?00鈩冩祦閫氳捀姹�15鍒嗛悩婊呰弻銆�

銆愯檿鏂瑰強宸ヨ棟鍒嗘瀽銆�

1.Vc锛氱儻浜岄唶绲�(ji茅)妲�(g貌u)锛岄’寮�(qi谩ng)閰告€�→pH瑾�(di脿o)绡€(ji茅)鍔�(纰抽吀姘垑/纰抽吀閳�)→閬垮厤鐤肩棝锛屽寮�(qi谩ng)绌�(w臎n)瀹氭€�

2.鏄撴哀鍖栨按瑙�→鎶楁哀鍔�(浜炵～閰告矮閳�)銆侀噾灞洟瀛愮怠(lu貌)鍚堝姂(EDTA-2Na)銆乸H瑾�(di脿o)绡€(ji茅)鍔�銆佸厖鎯版€ф埃楂�(婧舵恫涓€欳O2)

3.绌�(w臎n)瀹氭€ц垏婧害鏈夐棞(gu膩n)锛�100鈩冩祦閫氳捀姹芥粎鑿�15min

涓�銆佽几娑�

(澶у閲忔敞灏勫姂锛氭淮娉�>100ml)

(1)杓告恫鐨勫垎椤炶垏璩�(zh矛)閲忚姹�

(2)杓告恫鐨勫埗鍌欒垏璩�(zh矛)閲忔鏌�

(3)杓告恫涓昏瀛樺湪鐨勫晱(w猫n)椤屽強瑙ｆ焙鏂规硶

(4)鍏稿瀷杓告恫铏曟柟鑸囧埗鍌欏伐钘濆垎鏋�

(涓€)杓告恫鐨勫垎椤炶垏璩�(zh矛)閲忚姹�

1.杓告恫鐨勫垎椤炲強鑷ㄥ簥鐢ㄩ€�

鈶犻浕瑙ｈ唱(zh矛)杓告恫锛氳(b菙)鍏呴珨鍏�(n猫i)姘村垎銆侀浕瑙ｈ唱(zh矛)锛岀尘姝ｉ珨鍏�(n猫i)閰稿牽骞宠　绛夛紝姘寲閳�/寰�(f霉)鏂规隘鍖栭垑/涔抽吀閳�

鈶＄嚐(y铆ng)椁�(y菐ng)杓告恫锛氫笉鑳藉彛鏈嶅惛鏀剁嚐(y铆ng)椁�(y菐ng)锛岀硸/姘ㄥ熀閰�/鑴傝偑涔�

鈶㈣啝楂旇几娑诧細瑾�(di脿o)绡€(ji茅)楂斿収(n猫i)婊查€忓锛屽彸鏃嬬硸閰�/娣€绮夎鐢熺墿/鏄庤啝/PVP

鈶ｅ惈钘ヨ几娑�

2.杓告恫鐨勮唱(zh矛)閲忚姹傚強璩�(zh矛)閲忔鏌�——灏�(du矛)姣旀敞灏勫姂

√鐒�(w煤)鑿屻€佺劇(w煤)鐔卞師銆佹緞鏄庡害(鍙(ji脿n)鐣扮墿)——瑕佹眰鏇村姞鍤�(y谩n)鏍�

√瀹夊叏鎬�銆佹徊閫忓銆乸H銆佺┅(w臎n)瀹氭€�銆侀檷澹撶墿璩�(zh矛)

√涓嶅緱娣诲姞鎶戣弻鍔�

(浜�)杓告恫鐨勫埗鍌欏伐钘濇祦绋�

鍘熸枡鑸囪紨鏂�→绋遍噺→婵鹃亷(gu貌)→鐏岃→鍔犺啘→澹撹啝濉�→钃嬮媮钃�→鎵庨媮钃�→婊呰弻→璩�(zh矛)閲忔鏌�→璨兼(bi膩o)绨�→鍖呰→鎴愬搧

(涓�)杓告恫涓昏瀛樺湪鐨勫晱(w猫n)椤屽強瑙ｆ焙鏂规硶

3澶у晱(w猫n)椤�——鏌撹弻銆佺啽鍘�銆佸彲瑕�(ji脿n)鎬х暟鐗╄垏寰矑鍟�(w猫n)椤�

娉ㄥ皠娑蹭腑甯稿嚭鐝�(xi脿n)鐨勫井绮�——纰抽粦銆佺⒊閰搁垼銆佹哀鍖栭媴銆佺簴缍礌銆佺礄灞�銆侀粡鍦�銆佺幓鐠冨睉銆佺窗(x矛)鑿屽拰绲�(ji茅)鏅�

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铏曟柟

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2.闈滆剤娉ㄥ皠鐢ㄨ剛鑲钩

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鍥�銆佹敞灏勭敤鐒�(w煤)鑿岀矇鏈�(绮夐嚌)

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2.閬╀簬锛氬皪(du矛)婵曠啽鏁忔劅鐨勬姉鐢熺礌銆侀叾銆佽婕�

3.鍐峰噸骞茬嚗鍒跺搧锛氬喎鍑嶅共鐕�锛岀敓鐗╁埗鍝�(杓旈叾椤�)

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(2)婊寸溂鍔戣垏娲楃溂鍔�

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钘ョ墿鐨勫鍛ㄨ绠℃秷闄�

pH鑸噋Ka

鍒烘縺鎬�

琛ㄩ潰寮靛姏

榛忓害

QIAN锛氱溂鐨〃闈㈤粡鍊�(g猫)鍒�

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5.鐪肩敤娑查珨鍒跺姂

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